搶嫗堛壢帟壢戝妛丂擄帯幘姵尋媶強丂僎僲儉夝愅幒丂

Ion S5偑怴偨偵僎僲儉夝愅幒偵摫擖偝傟丄2018擭11寧傛傝庴戸夝愅傪奐巒偟傑偟偨丅

僎僲儉夝愅幒偵偼師悽戙僔乕僋僄儞僒乕Ion PGM偍傛傃Ion S5乮僒乕儌僼傿僢僔儍乕僒僀僄儞僥傿僼傿僢僋幮乯偑摫擖偝傟偰偍傝傑偡丅
埲壓偵Ion PGM偍傛傃Ion S5偵偮偄偰娙扨偵愢柧抳偟傑偡丅
傛傝徻嵶傪抦傝偨偄曽偼儊乕僇乕僒僀僩傪嶲徠偝傟傞偐丄僎僲儉夝愅幒偵偍栤偄崌傢偣壓偝偄丅

尰嵼僎僲儉夝愅幒偱偼埲壓偺庴戸夝愅僒乕價僗傪峴偭偰偍傝傑偡丅

(1)Ion PGM偍傛傃Ion S5偵傛傞僔乕僋僄儞僗乮Template嶌惉乣僔乕僋僄儞僗傑偱乯
(2)Ion僔乕僋僄儞僒乕梡儔僀僽儔儕偺嶌惢

懳墳壜擻側梡搑偼埲壓偺捠傝偱偡丅
丂

• 傾儞僾儕僐儞僔乕僋僄儞僗
• RNA-seq
• 慡僄僋僜儞僔乕僋僄儞僗
• ChIP-seq
• 儊僞僎僲儉夝愅

堦晹梡搑偼僔乕僋僄儞僗偺傒庴戸傪峴偭偰偍傝傑偡丅
徻嵶偼埲壓傪偛棗偔偩偝偄丅

Ion僔乕僋僄儞僒乕偵偮偄偰

丂Ion PGM偍傛傃Ion S5偼僒乕儌僼傿僢僔儍乕僒僀僄儞僥傿僼傿僢僋幮傛傝斕攧偝傟偰偄傞師悽戙僔乕僋僄儞僒乕偱偡丅堦夞偺儔儞偱挿偝200bp乣400bp偺攝楍傪500枩乣8000枩攝楍掱搙乮300M乣10G墫婎*乯摼傞偙偲偑壜擻偱偡丅僔乕僋僄儞僗偵巊梡偝傟傞夝愅僠僢僾偼暋悢偺庬椶偑偁傝丄偦傟偧傟夝愅偱偒傞墫婎悢偑堎側傝傑偡偺偱丄栚揑偵墳偠偰揔愗側傕偺傪慖戰偡傞偙偲偑壜擻偱偡丅
*M偼Mega偺棯丅1M墫婎=100墫婎丅G偼Giga偺棯丅1G墫婎=10壄墫婎丅

Ion僔乕僋僄儞僒乕偺僔乕僋僄儞僗尨棟

丂Ion宯偵尷傜偢師悽戙僔乕僋僄儞僒乕偼丄僒儞僈乕朄偵婎偯偄偨廬棃宆僔乕僋僄儞僒乕偲堎側傝僉儍僺儔儕乕傪巊梡偟側偄偙偲偱堦搙偵戝検偺攝楍夝愅傪幚尰偟偰偄傑偡丅Ion僔乕僋僄儞僒乕偺僔乕僋僄儞僗尨棟偼丄DNA偺怢挿斀墳偵敽偄惗惉偡傞僺儘儕儞巁傪専弌偡傞偙偲偱僔乕僋僄儞僗傪峴偆僷僀儘僔乕僋僄儞僗朄偵婎偯偄偰偄傑偡丅Ion僔乕僋僄儞僒乕偱偼僺儘儕儞巁偺惗惉傪pH偺曄壔偵傛傝専弌偟傑偡丅Ion僔乕僋僄儞僒乕偺夝愅僠僢僾偵偼悢昐枩乣悢愮枩偺彫偝側僂僃儖偑偁傝丄偦傟偧傟偺僂僃儖偑pH僙儞僒乕偲側偭偰偄傞偨傔丄堦夞偺儔儞偱戝検偺攝楍傪寛掕偡傞偙偲偑偱偒傑偡丅偨偩偟丄堦偮偺儕乕僪偵偮偒寛掕偱偒傞挿偝偼嵟戝偱400bp掱搙偱偡丅傑偨丄夝愅僠僢僾偵偼夝愅偟偨偄DNA傪崿崌偟偰棳偟崬傓偨傔丄桼棃偑堎側傞DNA傪摨帪偵夝愅偡傞応崌偼偦偺悢偵惂尷偑偁傝傑偡丅偙偺偨傔丄廬棃僎僲儉夝愅幒偵埶棅偝傟偰偄傞傛偆側僒儞僾儖傪摨帪偵悢昐枩僒儞僾儖夝愅偱偒傞傢偗偱偼側偄揰偵偛棷堄偔偩偝偄丅

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Ion僔乕僋僄儞僒乕偺僔乕僋僄儞僗庤弴

(1)儔僀僽儔儕挷惢
夝愅偟偨偄DNA偵丄Ion PGM傑偨偼Ion S5偱僔乕僋僄儞僗偡傞偨傔偺傾僟僾僞乕攝楍傪晅壛偟傑偡丅偙偺挷惍傪廔偊偨僒儞僾儖偼儔僀僽儔儕偲屇偽傟傑偡丅傾儞僾儕僐儞僔乕僋僄儞僗乮傾僾儕働乕僔儑儞偺崁嶲徠乯偺応崌偼PCR偵傛傞昗揑攝楍偺憹暆傕偙偺僗僥僢僾偵娷傑傟傑偡丅

(2)僥儞僾儗乕僩挷惍
旝嵶側悈揌偺拞偵價乕僘偲堦暘巕偺儔僀僽儔儕傪擖傟丄偦偺悈揌拞偱PCR傪峴偆偙偲偵傛傝堦偮偺價乕僘偵偮偒堦偮偺攝楍偑晅壛偟偨價乕僘傪挷惍偟傑偡乮僄儅儖僔儑儞PCR乯丅偙偺僗僥僢僾偼愱梡偺憰抲偱峴偄傑偡丅

(3)僔乕僋僄儞僗
夝愅僠僢僾偵挷惍偟偨僥儞僾儗乕僩傪棳偟崬傓偙偲偱僠僢僾忋偺奺僂僃儖偵堦偮偺價乕僘偑擖傝傑偡丅僔乕僋僄儞僗傪峴偆偲1僂僃儖偵懳偟偰堦偮偺攝楍偑摼傜傟傞偺偱丄1儔儞偱嵟戝悢愮枩偺攝楍傪摨帪偵寛掕偡傞偙偲偑壜擻偲側傝傑偡丅

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摉夝愅幒偱懳墳壜擻側傾僾儕働乕僔儑儞

傾儞僾儕僐儞僔乕僋僄儞僗

丂夝愅偟偨偄椞堟傪PCR偵傛傝憹暆偟偰僔乕僋僄儞僗偡傞庤朄偱偡丅悢昐乣悢愮庬椶偵媦傇憹暆嶻暔傪崿崌偟偨忬懺偱傑偲傔偰僔乕僋僄儞僗偟傑偡丅
丂傾儞僾儕僐儞僔乕僋僄儞僗偵巊梡偡傞僾儔僀儅乕僙僢僩偼丄埲壓偺愱梡僂僃僽僒僀僩偵堚揱巕柤傪擖傟傞偩偗偱僨僓僀儞偡傞偙偲偑壜擻偱偡丅傑偨丄50庬椶偺偑傫堚揱巕傪僞乕僎僢僩偲偡傞Cancer Hotspot Panel v2傗丄愭揤惈擄挳偵娭楢偡傞63堚揱巕傪僞乕僎僢僩偲偡傞Hearing Loss Research Panel摍丄懡悢偺僇僞儘僌僷僱儖偑僉僢僩偲偟偰斕攧偝傟偰偍傝傑偡丅
丂摉夝愅幒偱偼傾儞僾儕僐儞僔乕僋僄儞僗梡儔僀僽儔儕偺僔乕僋僄儞僗傪庴戸偟偰偍傝傑偡丅傑偨丄堦晹僷僱儖僉僢僩傪巊梡偟偨儔僀僽儔儕嶌惢傕庴戸偟偰偍傝傑偡丅僷僱儖偺庬椶偵偮偒傑偟偰偼偍栤偄崌傢偣偔偩偝偄丅

Ion AmpliSeq Desiner
https://www.ampliseq.com/login/login.action

RNA-seq

丂揮幨嶻暔傪僔乕僋僄儞僗偡傞偙偲偱堚揱巕偺敪尰検傪夝愅偟傑偡丅
Ion宯僔乕僋僄儞僒乕梡儔僀僽儔儕嶌惢僉僢僩偲偟偰丄梊傔僨僓僀儞偝傟偨僾儔僀儅乕偱僸僩偺20,802堚揱巕傪憹暆偡傞Ion AmpliSeq Transcriptome Kit偲丄mRNA傪僥儞僾儗乕僩偵儔僀僽儔儕傪嶌惢偡傞Ion Total RNA-seq Kit偑斕攧偝傟偰偄傑偡丅
丂摉夝愅幒偱偼椉帋栻偵傛傝嶌惢偝傟偨儔僀僽儔儕偺僔乕僋僄儞僗傪庴戸偟偰偍傝傑偡丅傑偨丄Ion AmpliSeq Transcriptome Kit偵傛傞儔僀僽儔儕嶌惢偺庴戸傕峴偭偰偍傝傑偡丅乮Ion AmpliSeq Transcriptome Kit偼儅僂僗偵傕懳墳偟偰偄傑偡丅乯

慡僄僋僜儞僔乕僋僄儞僗

丂僐乕僨傿儞僌椞堟偺DNA傪擹弅偟偰僔乕僋僄儞僗偟傑偡丅幘姵偺尨場堚揱巕傗偑傫懱嵶朎曄堎偺栐梾揑扵嶕側偳偵巊梡偝傟傑偡丅
丂Ion宯僔乕僋僄儞僒乕梡偺儔僀僽儔儕嶌惢僉僢僩偲偟偰丄僸僩偺19,000埲忋偺堚揱巕傪夝愅壜擻側Ion AmpliSeq Exome Kit偑偁傝傑偡丅
丂摉夝愅幒偱偼偙偺僉僢僩偵傛傞儔僀僽儔儕嶌惢偲僔乕僋僄儞僗偺庴戸傪峴偭偰偍傝傑偡丅

ChIP-seq

丂僋儘儅僠儞柶塽捑崀(ChIP)偵傛傝摼傜傟偨DNA傪僔乕僋僄儞僗偡傞偙偲偱丄僞儞僷僋幙偲DNA偺憡屳嶌梡傪栐梾揑偵夝愅偡傞曽朄偱偡丅揮幨場巕偺寢崌椞堟傗丄僸僗僩儞廋忺晹埵偺栐梾揑夝愅側偳偵棙梡偝傟傑偡丅
丂摉夝愅幒偱偼ChIP偵傛傝摼傜傟偨DNA偺儔僀僽儔儕嶌惢偲僔乕僋僄儞僗傪庴戸偟偰偍傝傑偡丅

儊僞僎僲儉夝愅

丂嵶嬠16S rRNA堚揱巕偺憹暆嶻暔傪僔乕僋僄儞僗偡傞偙偲偵傛傝丄嵶嬠憄偺慻惉傪掕検偡傞庤朄偱偡丅
Ion宯僔乕僋僄儞僒乕偱儊僞僎僲儉夝愅傪峴偆偨傔偺Ion 16S Metagenomics Kit偑斕攧偝傟偰偄傑偡丅
丂摉夝愅幒偱偼偙偺僉僢僩偱嶌惉偝傟偨儔僀僽儔儕偺僔乕僋僄儞僗傪庴戸偟偰偍傝傑偡丅


偦偺懠偺梡搑偵偮偒傑偟偰偼偛憡択偔偩偝偄丅

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