(1) 巹偨偪偼惗棟僗僩儗僗偵傛傞怴偟偄傾億僩乕僔僗宱楬傪採埬偟傑偟偨丅
We proposed a new pathway of physiological stress-induced apoptosis.
丂丂丂 徻 偟偔偼夋憸傪僋儕僢僋丂Click on for detail.
(2) 巹偨偪偼娻梷惂堚揱巕p53乮TP53)僼傽儈儕乕偺1偮偱偁傞p63(TP63)偑丄僀儞僥僌儕儞兛3僒僽儐僯僢僩傗MFG-E8偲偄偭偨嵶朎愙拝場巕偺堚揱巕敪尰傪桿摫偡傞偲偄偆怴偟偄奣擮傪帵偟傑偟偨丅
p63 (TP63), a member of the tumor suppressor p53 (TP53) family, is essential
for stratified epithelial development and tightly associated with squamous
cell carcinomas. Our results led to a new concept that p63 induces cell
adhesion molecules including integrin subunit alpha3 (ITGA3) and MFG-E8
(MFGE8) for proliferation.
J Biol Chem 2004, 279(48):50069-77; Oncogene 2008, 27(3):308-17.
(3) 巹偨偪偼丄夁姺婥偍傛傃嫊寣嵞娨棳偱偍偙傞攛忈奞偼TNF-alpha側偳偺僒僀僩僇僀儞傗ROS偺嶻惗丒梀棧偵傛傞偙偲丄傑偨偦傟傜梀棧場巕偑墦妘憻婍忈奞偺堦場偲側傝偆傞偙偲傪尒弌偟傑偟偨丅
儔僢僩梀棧攛儌僨儖 Isolated rat lung model
We can study different modes of lung injury by this system with the persta pump and respirator in combination with clip-1 and/or clip-2.
Clip-1: ischemia reperfusion
Clip-2: PEEP (positive and exspiratory pressure) and ZEEP (zero and expiratory
pressure)
(1) 嵶朎偺巁壔僗僩儗僗墳摎
Cellular responses to redox stress
嵶朎偺巁壔僗僩儗僗偺戝晹暘偼儈僩僐儞僪儕傾偺揹巕揱払宯乮屇媧嵔ATP嶻惗宯)偐傜偺妶惈巁慺(ROS)偺夁忚嶻惗偲僌儖僞僠僆儞傪娷傓儗僪僢僋僗挷愡宯偺婡擻晄慡偵傛傞丅巁壔僗僩儗僗偺忣曬偼僞儞僷僋幙儕儞巁壔宱楬偱戙昞偝傟傞僗僩儗僗僔僌僫儖揱払傪捠偠偰妀偵揱偊傜傟丄峈巁壔嶌梡傗嵶朎暘楐丄惗巰寛掕偵偐偐傢傞庬乆偺堚揱巕敪尰傪桿摫偡傞丅巹偨偪偼巁壔僗僩儗僗僔僌僫儖偵娭傢傞揮幨惂屼場巕偲昗揑堚揱巕偵拝栚偡傞丅傑偨巁壔僗僩儗僗墳摎偲偟偰偺丄嵶朎廃婜惂屼傗傾億僩乕僔僗桿摫偺暘巕婡峔傪尋媶偡傞丅尰嵼丄儈僩僐儞僪儕傾偺儊僞儃儕僘儉偲揹巕揱払宯偺妶惈偲ROS夁忚嶻惗偵傛偭偰捈愙惂屼偝傟傞傾億僩乕僔僗奐巒僇僗僷乕僛-9偺妶惈壔偵徟揰傪掕傔偰尋媶傪幚巤偟偰偄傞丅
(2) 滸暯忋旂娻嵶朎偵偍偗傞p63偺婡擻
Functions of the p63 proteins in squamous cell carcinomas
p63 (TP63)堚揱巕 偼娻梷惂堚揱巕p53 (TP53)僼傽儈儕乕乮p53丄p63丄p73乯偺堦堳偵悢偊傜傟丄p53偲椶帡偺峔憿傪帩偮揮幨挷愡場巕傪僐乕僪偟偰偄傞丅偟偐偟丄p63偼廳憌忋旂偺婎掙嵶朎丄偡側傢偪妏壔嵶朎乮働儔僠僲僒僀僩乯姴嵶朎偱摿堎揑偵敪尰偟丄摢栩晹傗旂晢偺崅暘壔宆偺滸暯忋旂娻偱崅敪尰偟偰偄傞丅巹偨偪偼滸暯忋旂娻偺敪徢偵偍偗傞p63偺婡擻傪柧傜偐偵偡傞偨傔偵丄(a) p63偺昗揑堚揱巕偺夝柧丄(b)
僔僌僫儖揱払宯傊偺塭嬁丄(c) p63堚揱巕帺懱偺敪尰挷愡丄(d) p63偺掅壓偵敽偆埆惈揮壔乮忋旂娫梩揮姺乯偺婡峔丄側偳傪夝愅偟偰偄傞丅
p63-expression in an epithelial dysplasia, immunohistochemistry,
Dr. Tsukinoki, K., Kanagawa Dental College
(3) 嫊寣嵞娨棳偵傛傞攛忈奞偺婡峔
Mechanisms of the lung injury by ischemia reperfusion
丂丂怱嬝峓嵡偵懳偡傞姤摦柆宍惉弍屻偍傛傃恖岺怱攛傪梡偄偨弍屻側偳丄懡婒偵傢偨傞昦懺偑丄嫊寣嵞娨棳忈奞偵傛傞偙偲偑抦傜傟偰偄傞丅偙偺忈奞偼丄嵞娨棳帪偵堦夁惈偵戝検偵敪惗偡傞妶惈巁慺(ROS)偵婲場偟丄僒僀僩僇僀儞偺嶻惗丄嵶朎撪Ca2+夁晧壸側偳偺槾恑偵傛傞偲峫偊傜傟傞丅傑偨嫊寣憻婍偵偲偳傑傜偢丄墦妘憻婍偵傑偱攇媦偟丄廳撃側忈奞乮懡憻婍晄慡乯傪傕偨傜偡応崌傕偁傞丅偟偐偟攛偼摿堎揑偱丄娨棳偑搑愨偊偰傕屇媧偵傛傞姺婥偑峴傢傟偰偄傞尷傝攛偵偼巁慺偑嫙媼偝傟傞偨傔偵丄嫊寣嵞娨棳忈奞偼婲偙傜側偄傕偺偲偝傟偰偒偨丅偲偙傠偑丄崱堜傜偑奐敪偟偨儔僢僩梀棧娨棳攛昗杮乮忋偺僴僀儔僀僩(3)嶲徠乯傪梡偄偰堦掕帪娫攛娨棳傪幷抐偡傞偲丄攛偵偍偄偰傕嫊寣嵞娨棳忈奞偑婲偙傞偙偲偑弶傔偰柧傜偐偵側偭偨丅偙偺帠幚偼丄攛愗彍帪偺曅攛姺婥偐傜椉攛姺婥偵栠偟偨嵺偵婲偙傞嵞朿挘惈攛悈庮側偳偺昦懺偑攛偺嫊寣嵞娨棳忈奞偱偁傞偙偲傪嫮偔帵偟偰偄傞丅
丂丂変乆偼丄偙偺儔僢僩梀棧娨棳攛昗杮傪梡偄偰攛偺嫊寣嵞娨棳偍傛傃夁姺婥偵傛傞攛忈奞偺暘巕婡峔偑ROS嶻惗偍傛傃僒僀僩僇僀儞偺堚揱巕敪尰偲嶻惗丒梀棧偵傛傞偙偲傪柧傜偐偵偡傞尋媶傪恑傔偰偄傞丅偝傜偵偙傟傜偺場巕偑墦妘憻婍忈奞偺尨場偲側偭偰偄傞壜擻惈傪専徹偟偰偄傞丅
憅揷弐堦丂Shun-ichi Kurata, Ph.D.
弝嫵庼丂 丂 Associate Professor, Medical Research Institute, TMDU
恄撧愳帟壢戝妛媞堳嫵庼丂Adjunct Professor, Kanagawa Dental College
僾儘僼傿乕儖
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尋媶栚揑丂
Aim of Research |
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妛楌偲尋媶楌丂
Education & Research Experiences
1973丂嬨廈戝妛棟妛晹
丂丂丂丂惗暔妛壢懖嬈丂
1980丂摨妛擾妛晹丂
丂丂丂丂攷巑壽掱廋椆擾妛攷巑
1980丂嶻嬈堛壢戝妛丂彆庤
1982丂棟壔妛尋媶強丂
丂丂丂丂棳摦尋媶堳
1984丂搶嫗堛壢帟壢戝妛
丂丂丂丂擄帯幘姵尋媶強丂彆庤
1993丂摨尋媶強丂彆嫵庼
2005丂摨尋媶強僼儘儞僥傿傾丒
丂丂丂丂儗僪僢僋僗墳摎嵶朎惗暔妛扴摉
丂丂丂丂
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丂
丂僸僩傗摦暔偼丄巁慺懚嵼壓偱惗懅偟偰偄傞偺偱巁慺偵傛傞嫮偄巁壔僗僩儗僗偵敇偝傟偰偄傞丅堦曽丄嵶朎撪巁壔僗僩儗僗偼庡偲偟偰儈僩僐儞僪儕傾偺揹巕揱払宯偐傜敪惗偡傞ROS(妶惈巁慺庬)偵婲場偡傞偲峫偊傜傟偰偍傝丄巁壔娨尦挷愡偲巁壔僗僩儗僗墳摎斀墳偼嵶朎偺惗懚偲儂儊僆僗僞僔僗偺偨傔偺晄壜寚側惗棟婡峔偱偁傞丅偙偺攋抅偵傛偭偰惗偠傞巁壔僗僩儗僗偼懡偔偺幘昦傗丄榁壔側偳偺尨場傑偨偼憹埆場巕偲偟偰嶌梡偡傞丅杮尋媶幒偱偼丄嵶朎撪僔僌僫儖揱払偲揮幨惂屼媦傃傾億僩乕僔僗桿摫側偳傪拞怱偵丄儈僩僐儞僪儕傾撪偺惗壔妛斀墳偐傜巒傑傞嵶朎偺儗僪僢僋僗墳摎宯偺暘巕婡峔偵娭偡傞尋媶傪峴偆偙偲傪捠偟偰懡偔偺巁壔僗僩儗僗偵娭學偡傞幘姵偺昦懺夝柧傪栚巜偡丅
丂丂傑偨丄憻婍偁傞偄偼屄懱儗儀儖偱偺ROS墳摎偲偟偰儔僢僩攛偺嫊寣嵞娨棳偍傛傃夁姺婥偵傛傞攛忈奞偺暘巕婡峔偺夝柧偲偲傕偵丄墦妘憻婍忈奞偺棟夝傪栚巜偟偰偄傞丅
丂丂偝傜偵丄嵶朎偺僔僌僫儖揱払尋媶偺敪揥偲偟偰丄娻梷惂僞儞僷僋幙p53僼傽儈儕乕偺堦堳偱偁傞p63偵偮偄偰丄滸暯忋旂娻嵶朎偵偍偗傞p63偺婡擻傗崅儗儀儖敪尰偺婡峔傪柧傜偐偵偟傛偆偲偟偰偄傞丅
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峺曣偵偍偗傞僄僗僩儘僎儞偺嶌梡丗丂嵶朎廃婜偺挷愡
Estrogen can regulate the cell cycle in丂the early G1 phase of yeast by increasing the丂amount of adenylate cyclase mRNA.
Tanaka S, Hasegawa S, Hishinuma F, Kurata S.
Cell. 1989 May 19;57(4):675-81.
巁壔僗僩儗僗偵傛傞揮幨場巕NF-kappaB偺妶惈壔: HIV LTR偺妶惈壔
Potential of azidothymidine to activate the HIV-1 promoter. Kurata, S. J Biol Chem. 1994 Oct 7;269(40):24553-6.
(Accelerated Publication)
Sensitization of the HIV-1-LTR upon long term low dose oxidative stress. Kurata, S.
J Biol Chem. 1996 Sep 6;271(36):21798-802.
掅儗儀儖巁壔僗僩儗僗偵傛傞僞儞僷僋幙儕儞巁壔僔僌僫儖揱払宯偺妶惈壔
Selective activation of p38 MAPK cascade and mitotic arrest caused by low level oxidative stress. Kurata, S.
J Biol Chem. 2000 Aug 4;275(31):23413-6.
(Accelerated Publication)
巁壔僗僩儗僗偵傛傞儈僩僐儞僪儕傾撪偱偺僇僗僷乕僛9偺妶惈壔偲傾億僩乕僔僗桿摫
Dimerization and processing of procaspase-9 by redox stress in mitochondria.
Katoh, I, Tomimori, Y, Ikawa, Y, Kurata, S.
J Biol Chem. 2004 Apr 9;279(15):15515-23. .
p63(偑傫梷惂堚揱巕p53僼傽儈儕乕)偵傛傞僀儞僥僌儕儞兛3堚揱巕偺揮幨妶惈壔偲儅僩儕僋僗傊偺愙拝桿摫
p51/p63 Controls subunit alpha3 of the major epidermis integrin anchoring the stem cells to the niche.
Kurata S, Okuyama T, Osada M, Watanabe T, Tomimori Y, Sato S, Iwai A, Tsuji
T, Ikawa Y, Katoh I.
J Biol Chem. 2004 Nov 26;279(48):50069-77.
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丂
暉惣嵷曚巕丂Nahoko Fukunishi, M.S.
僾儘僼傿乕儖
Profile |
妛夛敪昞
Presentation at Conferences |
妛楌丂Education
2005 撧椙愭抂壢妛媄弍戝妛堾戝妛丒廋巑壽掱廋椆
M.S., Nara Institute of Science and Technology (NAIST)
尋媶僥乕儅
Research for Ph.D. Thesis
滸暯忋旂娻嵶朎偺憹怋偵偍偗傞p63偺婡擻
Functions of p63 in proliferation of squamous cell carcinomas (SCCs).
滸暯忋旂偑傫嵶朎偱崅儗儀儖敪尰偡傞p63傪siRNA偱僲僢僋僟僂儞偟丄嵶朎廃婜丄傾億僩乕僔僗丄僞儞僷僋幙儕儞巁壔僔僌僫儖丄堚揱巕敪尰偺曄壔傪夝愅偟偰偄傑偡丅
p63 kockdown by siRNA transfection
Immunofluorescence staining of p63- knockdown cells for nuclear p63 (green)
and cytochrome c (red)
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滸暯忋旂娻嵶朎偱崅敪尰偡傞p63偵傛傞p53昗揑堚揱巕偺挷愡偲嵶朎憹怋
暉惣嵷曚巕丄壛摗埳梲巕丄敤棽堦榊丄憅揷弐堦
戞30夞擔杮暘巕惗暔妛夛丒戞70夞擔杮惗壔妛夛崌摨夛媍2007擭12寧14擔丂墶昹丂(4P-0803)
暉惣丂傎偐
戞67夞擔杮娻妛夛丂2008擭10寧28擔柤屆壆
暉惣丂傎偐
戞81夞擔杮惗壔妛夛丒戞31夞擔杮暘巕惗暔妛夛崌摨夛媍丂2008擭12寧9擔丂恄屗
丂丂丂
丂丂丂
丂丂丂丂丂Colony formation assay
Control siRNA-transfected cells (top) and p63-knockdown cells (bottom) grown in a soft agar medium.
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暯椦榓栫丂Kazuya Hirabayashi, M.D.
 丂2000 to present, in anti-chronological order
Zhu C, Bilali A, Georgieva GS, Kurata S,
Mitaka C, Imai T.
Salvage of nonischemic control lung from injury by unilateral ischemic
lung with apocynin, a nicotinamide adenine dinucleotide phosphate (NADPH)
oxidase inhibitor, in isolated perfused rat lung.
Transl Res. 2008 Dec;152(6):273-82. Epub
2008 Nov 14.
Katoh I, Sato S, Fukunishi N, Yoshida H,
Imai T, Kurata S.
Apaf-1-deficient fog mouse cell apoptosis involves hypo-polarization of
the mitochondrial inner membrane, ATP depletion and citrate accumulation.
Cell Res. 2008 Dec;18(12):1210-9.
Okuyama T, Kurata S, Tomimori Y, Fukunishi
N, Sato S, Osada M, Tsukinoki K, Jin HF, Yamashita A, Ito M, Kobayashi S,
Hata RI, Ikawa Y, Katoh I.
p63(TP63) elicits strong trans-activation of
the MFG-E8/lactadherin/BA46 gene through interactions between the TA and
DeltaN isoforms.
Oncogene. 2008 Jan 10;27(3):308-17. Epub 2007 Jul 16.
Georgieva GS, Kurata S, Ikeda S, Eishi Y,
Mitaka C, Imai T.
Nonischemic
lung injury by mediators from unilateral ischemic reperfused lung: ameliorating
effect of tumor necrosis factor-alpha-converting enzyme inhibitor.
Shock. 2007 Jan;27(1):84-90.
Georgieva GS, Kurata S, Ikeda S, Teng S,
Katoh I, Eishi Y, Mitaka C, Imai T.
Prevention of ischemia reperfusion injury by
positive pulmonary venous pressure in isolated rat lung.
Shock. 2006 Jan;25(1):66-72.
Watanabe T, Totsuka R, Miyatani S, Kurata
S, Sato S, Katoh I, Kobayashi S, Ikawa Y.
Production of the long and short forms of
MFG-E8 by epidermal keratinocytes.
Cell Tissue Res. 2005 Aug;321(2):185-93.
Epub 2005 Jun 11.
Kurata S, Okuyama T, Osada M, Watanabe T,
Tomimori Y, Sato S, Iwai A, Tsuji T, Ikawa Y, Katoh I.
p51/p63 Controls subunit alpha3 of the major
epidermis integrin anchoring the
stem cells to the niche.
J Biol Chem. 2004 Nov 26;279(48):50069-77. Epub 2004 Sep 12.
Katoh I, Tomimori Y, Ikawa Y, Kurata S.
Dimerization
and processing of procaspase-9 by redox stress in mitochondria.
J Biol Chem. 2004 Apr 9;279(15):15515-23. Epub 2004 Jan 27.
Tomimori Y, Katoh I, Kurata S, Okuyama T,
Kamiyama R, Ikawa Y.
Evolutionarily conserved expression pattern
and trans-regulating activity of Xenopus p51/p63.
Biochem Biophys Res Commun. 2004 Jan
9;313(2):230-6.
Teng S, Kurata S, Katoh I, Georgieva GS,
Nosaka T, Mitaka C, Imai T.
Cytokine mRNA expression in unilateral
ischemic-reperfused rat lung with salt solution supplemented with low-endotoxin or
standard bovine serum albumin.
Am J Physiol Lung Cell Mol Physiol. 2004
Jan;286(1):L137-42.
Yamaguchi T, Katoh I, Kurata S.
Azidothymidine causes functional and
structural destruction of mitochondria, glutathione deficiency and HIV-1 promoter
sensitization.
Eur J Biochem. 2002 Jun;269(11):2782-8.
Okada Y, Osada M, Kurata S, Sato S, Aisaki
K, Kageyama Y, Kihara K, Ikawa Y,
Katoh I.
p53
gene family p51(p63)-encoded, secondary transactivator p51B(TAp63alpha) occurs without forming an
immunoprecipitable complex with MDM2, but responds to genotoxic stress by accumulation.
Exp Cell Res. 2002 Jun 10;276(2):194-200. Highlight Paper
Katoh I, Aisaki KI, Kurata SI, Ikawa S,
Ikawa Y.
p51A (TAp63gamma), a p53 homolog, accumulates in response to DNA damage
for cell regulation.
Oncogene. 2000 Jun 22;19(27):3126-30.
Kurata S.
Selective activation of p38 MAPK cascade and
mitotic arrest caused by low level oxidative stress.
J Biol Chem. 2000 Aug 4;275(31):23413-6. Accelerated Publication
崱堜丂岶桽丂
慜搶嫗堛壢帟壢戝妛丒堛妛晹丒媬媫堛妛島嵗 嫵庼丂
(戝妛堾堛帟妛憤崌尋媶壢慡恖揑堛椕奐敪妛宯 慡恖恌抐帯椕妛島嵗乯
Takasuke Imai, M.D.
Former Professor, Critical Care Medicine, Tokyo Medical and Dental University
敤丂棽堦榊
恄撧愳帟壢戝妛丒岥峯惗壔妛島嵗丂嫵庼
岥峯擄帯幘姵僙儞僞乕挿
Ryu-Ichiro Hata, M.D., Ph.D.
Professor, Biochemistry and Molecular Biology
& Oral Health Science Research Center, Kanagawa Dental College
捨栘丂宐堦
恄撧愳帟壢戝妛丒岥峯擄帯幘姵僙儞僞乕丒暘巕昦棟丂嫵庼
Keiichi Tsukinoki, M.D.
Professor, Molecular Pathology, Oral Health Science Research Center,
Kanagawa Dental College
壛摗丂埳梲巕
嶳棞戝妛丒戝妛堾堛岺妛憤崌尋媶晹丂乮堛妛晹丒旝惗暔妛乯丂弝嫵庼
Iyoko Katoh, Ph.D.
Associate Professor, Microbiology, Interdisciplinary Graduate School of
Medicine & Engineering (Faculty of Medicine), University of Yamanashi
杮尋媶幒偱偼戝妛堾惗傪曞廤偟偰偄傑偡丅
搶嫗堛壢帟壢戝妛丒戝妛堾堛帟妛憤崌尋媶壢乮係擭娫偺攷巑壽掱乯
師偺傛偆側曽傪娊寎偟傑偡丅
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侾丆俀偵娷傑傟側偄曽傕偛憡択偔偩偝偄丅
楢棈愭:丂憅揷丂弐堦
 丂 乮仐乯傪@偵抲偒姺偊偰偔偩偝偄丅
kushbgen乮仐乯mri.tmd.ac.jp
03-5803-4695 尋媶幒捈捠
113-8510
搶嫗搒暥嫗嬫搾搰侾亅俆亅係俆
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